Modifying Enzyme Buffers
Sigma Aldrich Fine Chemicals Biosciences Pyruvate Kinase from rabbi
Pyruvate kinase from rabbit muscle catalyzes an ATP-dependent phosphorylation of glycolate to yield 2-phosphoglycolate.
Boston Bioproducts Inc Kinase Extraction Buffer 500ml
Kinase Extraction Buffer - 500ml (For Research Use and Further Manufacturing Only)
Apexbio Technology LLC Phosphatase Inhibitor Cocktail 3 (100X in DMSO) 100x1ml
Numerous cellular processes are regulated by reversible protein phosphorylation with approximately 30% of proteins being phosphorylated during their lifespan Crude cell extracts contain various endogenous enzymes such as phosphatases and proteases which can degrade and alter proteins in the extracts Therefore the optimal method to increase the yield of intact proteins is to add inhibitors targeting these enzymes Phosphatase Inhibitor Cocktail 3 inhibits alkaline phosphatase L-isozymes and serine/threonine protein phosphatases such as protein phosphatase 1 and 2A (PP1 and PP2A) Phosphatase Inhibitor Cocktail 3 has been optimized and tested in cell extracts from various animal tissues Phosphatase Inhibitor Cocktail 3 can replace Phosphatase Inhibitor Cocktail 1 as the two cocktails have equivalent activities Phosphatase Inhibitor Cocktail 3 contains Cantharidin Bromotetramisole and Calyculin A This Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO
Revvity Health Sciences Inc HTRF Enzymatic Kinase Buffer (5X), 10 mL
HTRF Enzymatic Kinase Buffer (5X), 10 mL
Boston Bioproducts Inc 10X qPCR Proteinase K 100ml
qPCR Proteinase K Buffer (10X) - 100ml (For Research Use and Further Manufacturing Only)
Revvity Health Sciences Inc HTRF Stimulation Buffer #2 (5X), 8 mL
HTRF Stimulation Buffer #2 (5X), 8 mL
Revvity Health Sciences Inc HTRF Stimulation Buffer #1 (5X), 8 mL
HTRF Stimulation Buffer #1 (5X), 8 mL
Revvity Health Sciences Inc HTRF Phospho and Total protein blocking reagent, 6 ml
HTRF Phospho and Total protein blocking reagent, 6 ml
New England Biolabs, Inc. StickTogether™ DNA Ligase Buffer – 2 ml
New England Biolabs supplies a reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of T3, T7, Hi-T4 and Salt-T4 DNA Ligases .